Jul 11, 2017 a synovial fluid analysis is performed when pain, inflammation, or swelling occurs in a joint, or when theres an accumulation of fluid with an unknown cause. Synovial fluid is a viscous, nonnewtonian fluid found in the cavities of synovial joints. In particular, rheopexy, as a consequence of aggregation under shear, is observed in important biological fluids such as protein solutions e. Over 80% of people over the age of 60 show radiographic evidence of oa. Tribological and rheological properties of a synovial fluid model. Rheopexy of synovial fluid and protein aggregation nist. Since rheopexy is observed for the synovial fluid model, rheopexy must be caused by the macromolecules in synovial fluid.
Investigation of protein content of synovial fluids with dsc. The objective was to investigate the role of proteins. Rheopexy of synovial fluid and protein aggregation journal. The objective was to investigate the role of proteins in th. Measurement of protein denaturation in human synovial fluid. Osteoarthritic synovial fluid rheology and correlations with. Oa synovial fluid samples were pooled and subjected to depletion of abundant proteins by mars6 lc column and lectin affinity chromatography using three different lectins concanavalin a, wheat germ agglutinin and jacalin. However, rheological differences between sf and pure ha solutions suggest that sf proteins actively contribute towards the bulk viscoelasticity of this biological fluid. A tomato juice containing 1% soy protein demonstrates a rheopectic behavior at shear rates lower than 250 s. This unusual flow characteristic is caused by protein aggregation, and the total stress is enhanced by entanglement of this tenuous protein network with the longchain polysaccharide sodium hyaluronate under physiological conditions. Rheopexy of synovial fluid and protein aggregation. Knowing that the synovial fluid contains different blood plasma proteins in high amount 2, 16,17,18, we have decomposed the scans with gaussian functions to demonstrate the effect of severity of the disease or infections, see fig. Sep 05, 2005 protein aggregation appears to play an important role in the mechanical properties of blood and synovial fluid.
Citeseerx rheopexy of synovial fluid and protein aggregation. Research article enrichment of extracellular vesicles from human synovial fluid using size exclusion chromatography andrew d. Sheardriven activation, aggregation, and rheopexy in sheared. Dawson from the department of ophthalmology, college of physicians. A synovial fluid and bovine serum albumin bsa solution demonstrates rheopectic behavior at shear rates below 10. It is known that the concentration of the synovial fluid in the protein. Critical examination of the colloidal particle model of. Above this critical shear rate and up to at least 70. This fraction was eluted in the void volume of a biogel a5m column, gave a single precipitin line both with antisera to synovial fluid. We also suggest a connection between the observed rheopexy and the remarkable. However, compared to the newtonian behavior of the lubricants studied here, synovial fluid has been shown to. A total of 20 patients were divided into two groups with 10 patients in each group. In the case of joint diseases, as a result of the process induced by the pathophysiological. Pdf rheopexy of synovial fluid and protein aggregation.
Effects of dilution and intermolecular interactions simon j. This unusual flow characteristic is caused by protein aggregation, and the total stress is enhanced by entanglement of this tenuous protein network with the longchain polysaccharide sodium hyaluronate. The picture shows initial stages of self organisation of the paenibacillus dendritiformis bacteria from droplet inoculation at the bottom left. With its yolklike consistency, the principal role of synovial fluid is to reduce friction between the articular cartilage of synovial joints during movement. With its egg whitelike consistency, the principal role of synovial fluid is to reduce friction between the articular cartilage of synovial joints during movement.
The elevation of serum uric acid in cases of gout is well known. After aggregation had been accomplished, proteins were measured by the lowry method utilizing a yglobulin standard with a known protein concentration breakdown products of c3 in human synovial. Tribological and rheological properties of a synovial fluid. Work with medimmune scientists and penn state graduate students on models for protein aggregation. Tribological and rheological properties of a synovial fluid model rebecca klossner, jing liang, wendy krause, north carolina state university hyaluronic acid ha and the plasma proteins, albumin and globulins, are the most abundant macromolecules in synovial uid, the uid that lubricates freely moving joints. Further, rheopexy may even lead, under certain conditions, to a liquidsolid. This unusual flow characteristic is caused by protein aggregation. Rheology of synovial fluid with and without viscosupplements. Proteomic analysis of human osteoarthritis synovial fluid. In contact observation of model synovial fluid lubricating mechanisms in contact observation of model synovial fluid lubricating mechanisms myant, connor. Dawson from the department of ophthalmology, college of physicians and surgeons. The results show that, at the synovial fluid concentrations tested, the protein content plays a negligible role in either the fluid s bulk shear or extensional flow behaviour.
Sheardriven activation, aggregation, and rheopexy in. The rheological properties of synovial fluid sf are largely attributed to the presence of high molecular weight hyaluronan ha. Apr, 2010 differential scanning calorimetry dsc was used to evaluate the thermal transitions associated with protein constituents of synovial fluid samples from three individuals with osteoarthritis. Rheological study of healthy and pathological synovial fluid after. This paper reports a fundamental study of lubricant film formation with model synovial fluid components proteins and bovine serum bs. Investigation of protein content of synovial fluids with. This cited by count includes citations to the following articles in scholar.
The volume of synovial fluid was found to be increased in dogs with joint disorders when compared to the synovial fluid volume in normal dogs which is in acco rdance with the earlier reports of where 520 ml of synovial fluid was recorded in dogs affected with elbow or stifle pathology6. Therefore, this study aimed to identify the concentration changes in the synovial fluid total protein amount and individual proteins associated with suprapatellar bursitis using the bradford protein assay and western immunoglobulin methods. Rheological effects of macromolecular interactions in. Rheology of synovial fluid is of interest because of its significance in the joint lubrication. Protein aggregation appears to play an important role in the mechanical properties of blood and synovial fluid. Synovial fluid analysis in the diagnosis of joint disease. Figure 3 shows that bsa aggregates under physiological conditions and it is likely that other globular proteins globulins, etc.
Work flow illustrating the steps involved in the proteomic analysis of oa synovial fluid. Rheological study of viscosupplements and synovial fluid in. In contact observation of model synovial fluid lubricating. Investigation of protein content of synovial fluids with dsc in. Film thickness was measured by optical interferometry in a ballondisc device mean speed range of 260 mms. The nature and amount of precipitate varies from good to poor and re. Joint conditions like arthritis, gout, infections, and bleeding disorders can change. The extremely low friction and minimal wear in natural synovial joints appear to be established by effective lubrication mechanisms based on appropriate combination of articular cartilage and synovial fluid. The ones marked may be different from the article in the profile.
Synovial fluid orthopaedicsone articles orthopaedicsone. The presence of lowgrade inflammation in oa joints is receiving increasing attention, with synovitis shown to be present even in the early stages of the disease. Recent studies of globular protein solutions have uniformly adopted a colloidal view of proteins as particles, a perspective that neglects the polymeric primary structure of these biological. C and can be resolved into three peaks, likely due to the unfolding. Theory of activatedrate processes under shear with. Synovial fluid samples were collected from 20 ra patients. Rheology, viscosupplements, osteoarthritis, synovial fluid. Schematic of the work flow implemented in the study. The research showed model synovial fluid solutions formed much thicker films than expected which was attributed to protein agglomeration close to the contact. Enrichment of extracellular vesicles from human synovial. Osteoarthritis oa is a degenerative disease characterized by cartilage breakdown in the synovial joints. Biomimetics is an emerging field of research in engineering and successes have been documented in the diverse fields of robotics, mechanics, materials engineering, and many more. Bovine synovial fluid has similar viscosity values. A new mechanism for lubricating film formation with model synovial fluids.
We also suggest a connection between the observed rheopexy and the remarkable lubrication properties of synovial fluid. Synovial fluid proteome in rheumatoid arthritis clinical. Human synovial fluid the joint fluid was aspirated from knee joints of patients with rheuma toid arthritis 1, 2. Rheopexy of synovial fluid and protein aggregation, j. The objective was to investigate the role of proteins in the lubrication process. Increased levels are found in inflammatory and hemorrhagic disorders. Metals in the forms of protein bound metal ions or nanosized particles can also increase protein protein interactions and aggregation, which can cause some health effects and change the. Synovial fluid analysis definition of synovial fluid. How the synovial inflammation arises is unclear, but proteins in the synovial fluid of affected joints could conceivably. Synovial fluid is an ultrafiltrate from plasma, and contains proteins derived from the blood plasma and proteins that are produced by cells within the joint tissues. The highviscosity film is due to inlet aggregation of protein molecules forming a gel which is entrained into. Osteoarthritis oa is a degenerative joint disease that is characterized by the breakdown of articular cartilage.
The inner membrane of synovial joints is called the synovial membrane and secretes synovial fluid into the joint cavity. Upon shearing, the structure of the confined synovial fluid changes dramatically when sheared above a critical shear rate of about 2 s. A synovial joint fluid analysis is a group of tests your doctor can use to diagnose problems with your joints. This model, recently proposed by the authors, suggests that interfacial film formation is determined by rheological changes local to the contact and is driven by aggregation of synovial fluid proteins. Journal of the royal society interface 2006, 3 6, 167174. Rheopexy of synovial fluid and protein aggregation rheopexy of synovial fluid and protein aggregation. The healthy synovial fluid curve was similar to the healthy blood plasma scans. This helps support the validity of the analysis of synovial fluid ha content, either by microfluidic or by other techniques where the synovial fluid. Suekawa, kotaro tanimoto, yuki tanne, tomomi mitsuyoshi, naoto hirose, shaoching su, kazuo tanne and eiji tanaka, synthesis of hyaluronan and superficial zone protein in synovial membrane cells modulated by fluid flow, european journal of oral sciences, 121, 6, 566572, 20.
It seems to furnish a beautiful example of flowinduced aggregation that involves the subtle interaction of polysaccharides and proteins. Role of proteins in the degradation of relatively inert. The tribology of the sfm was also investigated using nanoindenterbased scratch tests. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Tribological and rheological properties of a synovial. Naha selfassociations, protein aggregation, proteins binding. Pdf bovine synovial fluid and albumin solutions of similar concentration are rheopectic stress increases with time in steady shear. Damage to the synovial membrane produces pain and stiffness in the joints that cause what. These papers tell us that rheological behavior is complex and many factors affect this phenomenon, e. Synovial fluid, also called synovia, is a viscous, nonnewtonian fluid found in the cavities of. Colby, rheopexy of synovial fluid and protein aggregation. Rheopexy definition of rheopexy by medical dictionary. This helps support the validity of the analysis of synovial fluid ha content, either by microfluidic or by other techniques where the synovial fluid is first diluted, and suggests that the ha and protein content in synovial fluid must be higher than a certain minimum threshold concentration before ha protein or protein protein interactions. Naha selfassociations, protein aggregation, proteins binding to naha, or some combination of the three.
Nature shows numerous examples of systems which show energy efficiency, elegance in their design and optimum use of materials. Sfa is commonly performed on younger pts to detect infectioneg. Bovine synovial fluid and albumin solutions of similar concentration are rheopectic stress increases with time in steady shear. Equal amounts of proteins were taken from all samples and pooled together followed by two sets of protein enrichment. The protein concentration of sf as well as its intraarticular production and decrease in normal equilibrium is determined by synovial blood flow, plasma concentration, microvascular permeability, and lymph node separation.
Synovial fluid lubrication is dependent on protective protein films that form between joint surfaces. Rheopexy of synovial fluid and protein aggregation core. This unusual flow characteristic is caused by protein aggregation, and the total stress is enhanced by entanglement of this tenuous protein network. Tribological and rheological properties of a synovial fluid model rebecca klossner, jing liang, wendy krause, north carolina state university hyaluronic acid ha and the plasma proteins, albumin and globulins, are the most abundant macromolecules in synovial. The observed rheopexy and shear history dependence are indicative of structure building in solution, which is most likely caused by protein aggregation. Synovial fluid, also called synovia, is a viscous, nonnewtonian fluid found in the cavities of synovial joints. Synovial fluid is an ultrafiltrate from plasma, and contains proteins derived from the. Jul 01, 20 read in contact observation of model synovial fluid lubricating mechanisms, tribology international on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. However, there are still many questions related to synovial fluid rheology and its relation with oa. First, the endothelial lining of the capillaries is traversed fol lowed by movement through a matrix that surrounds syn ovial cells.
Plasma proteins present in osteoarthritic synovial fluid can. Figure 112 shows the synovial lining of the synovial capsule. The objective was to investigate the role of proteins in the. Biomolecules free fulltext rheopectic behavior for.
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